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Effect of microRNA10a on migration and invasion of gastric cancer cell line BGC823
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Abstract:
Abstract Objective: To investigate the effect of human microRNA10a(miR10a) on the migration and invasion of gastric cancer cell line BGC823. Methods: The Transwell system was used to select highly invasive subcell lines from gastric cancer cell line BGC823. Using miRNA microchip, we compared the miRNA expression in paired cell lines with high and low invasive potentials. MiR10a was relatively overexpressed in the highly invasive cell lines when compared with its counterpart. The mature type human miR10a was synthesized chemically. The synthesized miR10a（25, 50, 100, and 150 nmol/L）was transfected into BGC823 cells via lipofectamin 2000. Cells were also transfected with empty vectors and unrelated fragment to serve as controls. The expression of mature type miR10a was detected by realtime PCR. Cell counting kit8 was used to study the effect of miR10a on the proliferation of BGC823 cells. Flow cytometry was performed to detect the effect of miR10a on the apoptosis of BGC823 cells. The migration and invasion of BGC823 cells were investigated by Transwell assay. Results: Realtime PCR showed that cells transfected with mature type miR10a had significantly higher expression of miR10a, with the optimal concentration of miR10a being 100 nmol/L; the associated miR10a expression was 2.06 folds that of unrelated group. miR10a (100 nmol/L) had no obvious influence on the proliferation and apoptosis of BGC823 cells; however, it promoted the migration and invasion of BGC823 cells, with the promoting rates being (88.34±0.61)% and (56.02±3.13)%, respectively. Conclusion: Synthesized mature type human miR10a can effectively enhance miR10a expression and promote the migration and invasion of the BGC823 cells.
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Project Supported:
Supported by the National Natural Science Foundation of China（No. 30570818）; the Major State Basic Research and Development Program (973) of China（No.2009CB521804）
Clc Number:
