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Human osteosarcoma multidrug resistancerelated protein identified by differential ingel electrophoresis
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Abstract:
Abstract Objective: To analyze the differential protein expression between multidrugresistant human osteosarcoma MG63/DXR100 and its parental cell line by differential ingel electrophoresis, so as to lay a foundation for exploring the mechanism of multidrug resistance(MDR) of osteosarcoma. Methods: Multidrugresistant clone of human osteosarcoma MG63 was established by stepwise exposure to increasing doses of doxorubicin(DXR). The total proteins of the above two cell lines were separated with twodimensional electrophoresis. The proteins of differential expression (increased or decreased by more than 30%) were analyzed with image scanning and DeCyder software. Then they were identified in MALDITOF Pro and Mascot database. Results: Thirty proteins with differential expression were identified by proteomic analysis, and 18 of them were further identified by MALDITOF Pro. Five protein spots were successfully identified: matrix metalloproteinases 1 (MMP1) related with tumor cell metastasis, alcohol dehydrogenase (ADH6) with function of detoxication, FERM domain containing protein 3 (FRMD3) which belongs to antioncogenes and two agnoproteins composed of 128 and 300 amino acid residues. MMP1, ADH6 and the two agnoproteins were overexpressed in MG63/DXR100 group, and FRMD3 expression was lower than that in the MG63 group. Conclusion: Five proteins including MMP1, ADH6, FRMD3 and two agnoproteins have been found abnormally expressed in MDR osteosarcoma cells by differential ingel electrophoresis; they might participate in MDR of osteosarcoma.
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Project Supported:
Supported by the National Natural Science Foundation of China (No. 30772210)
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