Mobile website
Basic Research
Inhibitory effect of 4′methyletherscutellarein on human choriocarcinoma JAR cells and the possible mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Abstract Objective: To investigate the inhibitory effect of 4′methyletherscutellarein (4MS), an extract from Verbena officinalis, on human choriocarcinoma JAR cell line and the possible mechanism. Methods：JAR cells were exposed to 4′methyletherscutellarein of different concentrations for 48 h. MTT assay was used to examine the antiproliferative effect of 4′methyletherscutellarein. Flow cytometry was used to investigate the apoptosis and the changes of cell cycle. AO/EB double staining was applied to discriminate the apoptotic cells from dead ones. The changes of Survivin, p38MAPK and Caspase 3mRNA expressions were detected by RTPCR in JAR cells treated with 4MS. Furthermore, Western blotting assay was used to determine Survivin protein expression, phosphorylation level of p38 and Caspase 3 in JAR cells before and after 4MS treatment. Results：4MS inhibited the proliferation of JAR cells in a dose and timedependent manner (P<0.05,P<0.01). 4MS treatment also induced apoptosis in JAR cells in a concentrationdependent manner, and percentage of cell cycle progression in G2/M phase increased dramatically compared with the control group (P<0.05). The result of AO/EB double staining showed that there were more viable apoptotic cells in 4MS treated groups than in the control group and the number of nonviable apoptotic cells and dead cells increased with dose. Phosphorylated p38 and Caspase 3 expressions in 4MS treated cells were increased at both mRNA and protein levels according to RTPCR and Western blotting results, while Survivin expression was downregulated; there were significant differences between the 4MS group and the control group (P<0.05). Conclusion：4MS can inhibit proliferation of JAR cells and induce their apoptosis, which might be related to cell arrest at G2/M, downregulation of Survivin activity, and direct activation of p38 MAPK pathway and Caspase 3.
Keywords:
Project Supported:
Supported by the Natural Science Foundation of Jiangsu Province(No.BK2003104)
Clc Number:
