Mobile website
Basic Research
Matrine induces apoptosis of glioma cell C6 and the possible mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Abstract Objective: To explore the apoptosis inducing effect of matrine on C6 glioma cells and the related mechanisms. Methods: MTT assay was used to examine the inhibition of C6 glioma cell line by matrine at various concentrations and the IC50 was calculated. Inverted microscope and TEM (transmission electron microscope) were employed to observe the morphological alterations of C6 glioma cells after exposure to matrine; FCM (Flow cytometry) was used to detect the apoptosis rate of C6 glioma cells; and realtime PCR was used to examine the differential expression of related genes. ICC and Western blotting was used to detect the expression of caspase3. Results: MTT showed that the cell inhibition effect of matrine increased with its concentration（0.11.0 mg/ml）（P<0.05）, with the IC50 being 0.715 mg/ml. Inverted microscope and TEM showed that matrine induced apoptosis of C6 glioma cells. FCM showed that the apoptosis rate increased[(3.56±0.73)%-(27.55±1.92)%] with the increase of matrine concentration（0.2-1.0 mg/ml）（P<0.05）. Realtime PCR showed that matrine induced upregulation of 58 genes and downregulation of 11 genes, and 22 of them were related to apoptosisinducing death receptor, 15 of them to apoptosisinducing mitochondrial pathway. ICC and Western blotting showed that matrine induced upregulation of caspase3 expression（P<0.05）. Conclusion: Matrine can induce C6 glioma cell apoptosis and the mechanism might be related to a number of genes involved in death receptor pathway and mitochondrial pathway.
Keywords:
Project Supported:
Supported by the Natural Science Foundation of Heilongjiang Province (No.D 200739)
Clc Number:
