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Influence of glypican3 gene on proliferation，adhesion and invasion of hepatoma carcinoma cancer cell line SKHep1
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Abstract:
Abstract Objective: To study the influence of GPC3 gene on the proliferation, adhesion and invasion of hepatoma cell line SKHep1. Methods: SKHep1 cells were transfected with pEGFPN2GPC3 using Lipofectamine2000. RTPCR was used to examine the GPC3mRNA expression in GPC3SKHep1 cells. MTT assay was used to examine the proliferation and calculate the adhesion rate of SKHep1 cells. Transwell system was used to assess the migration and invasion of the cells. Results: SKHep1 cells were successfully transfected with pEGFPN2GPC palsmid. GPC3 mRNA was detected in SKHep1 cells. Transfection with CPC3 significantly suppressed the growth of SKHep1 cells（P<0.01）. The adhesion ability of GPC3transfected cells was significantly decreased compared with control group（ [10.21±0.62]% vs [15.51±0.95]%, P<0.01]. Transfection with GPC3 significantly enhanced migration and invasion capacity（[131.7±7.44] vs [69.6±5.25]，P<0.01; [220±12.8] vs [130±8.2]，P<0.01]. Conclusion: Transfection with GPC3 gene can greatly inhibit the proliferation and adhesion of hepatoma SKHep1 cells, but can enhance their migration and invasiveness. The present study provides an experimental basis for studying the invasion mechanism of liver cancer.
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Project Supported:
Supported by the Young Technological Talents Innovative Projects of Fujian Provience (No.2005J074)
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