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Effect of trichostatin A and paclitaxel on growth and apoptosis of endometrial carcinoma cells
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Abstract:
Objective:To investigate the effect of trichostatin A (TSA) and paclitaxel (PTX) on the growth and apoptosis of human endometrial carcinoma KLE cell line. Methods: KLE cells were cultured in the presence of PTX, doxorubicin, carboplatin, histone deacetylase inhibitor TSA or their combination. The growth curve was obtained by trypanblue exclusion assay and cell counting. Cell apoptosis was observed by Annexin V, Hoechst staining and perturbation of mitochondrial membrane potential. The protein expression of PARP, caspase9 and tubulin acetylation was detected by Western blotting. Results: Paclitaxel, doxorubicin, carboplatin, and TSA all significant inhibited the growth of KLE cells. PTX and TSA both induced cell apoptosis; the combined treatment with TSA and PTX induced more severe apoptosis. Western blotting and immunohistochemistry analysis demonstrated that TSA and PTX induced acetylation of tubulin, and a combination of both resulted in more severe acetylation of tubulin. Conclusion: The synergy of TSA and PTX can greatly inhibit the growth of KLE cells and induce their apoptosis.
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Project Supported:
Supported by the Importing Overseas Intelligence Program Foundation of Shandong Province（No.20083700340）
Clc Number:
