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CpG ODN107 enhances radiosensitivity of human nasopharyngeal carcinoma cell line CNE2 to βray in vitro
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Abstract:
Objective:To screen for specific CpG ODN sequence which can enhance the radiosensitivity of nasopharyngeal carcinoma cell line CNE2 cells to βray and to observe the sensitivityenhancing characters of the screened CpG ODN sequence, so as to provide evidence for searching novel sensitizer of nasopharyngeal carcinoma to radiation. Methods: MTT assay was used to select the strongest sequence to sensitize CNE2 cells to βray irradiation from 21 CpG ODN sequences. The effect of the selected sequence combined with βray irradiation were used to treat CNE2 cells and the proliferation, colony forming, cell migration, cell cycle and apoptosis were all observed. Results: Screen assay found CpG ODN107 had the highest SER (1.59±0.06) for CNE2 cells. CpG ODN107 combined with βray irradiation inhibited CNE2 cell proliferation in a dose-dependent manner, and the inhibitory effect was significantly higher than that of radiation alone (P<0.05, P<0.01). CpG ODN107 combined with βray irradiation significantly inhibited the formation and migration of CNE2 cell colony, and induced G0/G1 arrest and significantly increased cell apoptosis compared with that of radiation alone (P<0.05 or 0.01). Conclusion：Our results suggest that CpG ODN107 can greatly enhance the radiosensitivities of human nasopharyngeal carcinoma cell line CNE2 to βray radiation, which might be related to the induction of cell cycle arrest and apoptosis.
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Project Supported:
Supported by the National Natural Science Foundation of China（No.30271512）
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