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Influence of Bifidobacterium on expression of HIF1α and VEGF in rabbit VX2 tumor
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Abstract:
Objective:To investigate the influence of Bifidobacterium adolescence on the expression of HIF1α and VEGF in rabbit VX2 tumor. Methods: Rabbit VX2 tumor models were established and were divided into two groups (n=10). Animals in the experimental group were injected with Bifidobacterium adolescence and those in the control group received normal saline via ear vein. Animals were sacrificed and the liver, kidney, spleen, heart, lung and tumor tissues were removed, cultured and Gram stained. The changes of tumor tissue were observed by HE staining; immunohistochemistry was used to detect the expression of HIF1α and VEGF in tumor tissues. Results:Bifidobacterium adolescence could target the tumor tissue. HE staining showed the necrotic area was obviously enlarged after treatment with Bifidobacterium. Immunohistochemistry demonstrated that the positive rates of HIF1α and VEGF in the experimental group were significantly lower than those of the control group(P<0.05), and the expression of the HIF1α and VEGF in two groups was positively correlated (r=0.86). Conclusion:Bifidobacterium adolescence can specifically target rabbit VX2 tumors tissues, and decrease the expression of HIF1α and VEGF.
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Project Supported:
Suppording by the Doctoral Research Foundation from Science and Technology Commission of Shandong Province（No. 2006BS03035）
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