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targeted siRNA combined with TRAIL induces apoptosis of breast cancer cell line MCF7
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Abstract:
Objective:To investigate the promoting effect of silencing mitogenactivated protein/ERK kinase kinase 3 ( MEKK3 )gene on TRAIL-induced apoptosis of breast cancer MCF7 cells, so as to search for a novel clinical treatment strategy for breast cancer. Methods: TRAIL was used to treat MCF7 cells and the growth inhibition of MCF7 cells was determined by a methyl thiazolyl tetrazolium ( MTT ) assay．Chemically synthesized small interfering RNA ( siRNA ) targeting MEKK3 was transfected into MCF7 cells using DharmaFECT Transfection reagent, and the expression of MEKK3mRNA and protein was detected by RTPCR and Western blotting. The proliferation and apoptosis of MCF7 cells were analyzed by MTT and flow cytometry ( FCM ) after treatment with MEKK3siRNA combined with TRAIL．Results: TRAIL inhibited the proliferation of MCF7 cells, but the inhibitory effect was weak. Transfection with MEKK3siRNA effectively and stably inhibited the expression of MEKK3mRNA and protein expression (P<0.01). Combination of TRAIL and MEKK3siRNA more severely inhibited the proliferation of MCF7 cells compared with TRAIL alone (P<0.05); besides, the combination also increased the apoptosis rate of MCF7 cells (P<0.05). Conclusion: Silencing of MEKK3gene with siRNA can greatly promote TRAILinduced apoptosis of breast cancer cells MCF7, which lays an experimental foundation for new treatment method of breast cancer.
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Project Supported:
Supported by the Science Development Program of Shanghai Jiaotong University(No.05xj21022)
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