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NogoB expression in human brain gliomas and its relationship with tumor pathological grades
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Abstract:
Objective: To explore the expression of NogoB in brain gliomas and its relationship with the pathological grades of gliomas. Methods: The expression of NogoB was investigated in 10 normal brain tissues and 36 brain glioma specimens, which were confirmed after surgery during 2005-2008 in Changzheng Hospital, using immunohistochemical method, realtime PCR and Western blotting assay．The relationship between NogoB expression and pathological grades were analyzed. Results:Tissue NogoB immunohistochemical staining in gliomas specimens suggested the involvement of NogoB in the proliferation of gliomas. The realtime PCR demonstrated that NogoB mRNA expression level in gliomas was obviously decreased in gliomas of high pathological grades, and the expression was stable in normal brain tissues. The expression of NogoB was negatively correlated with the malignancy of glioma. The expression in gliomas of grade Ⅲ decreased by 90% compared with the normal brain tissues (P<0.01). Western blotting showed that NogoB protein expression was stable in normal brain tissues and obviously decreased in glioma tissues of high grade; the decrease was negatively correlated with the malignancy of gliomas. Immunohistochemical analysis showed NogoB expression was scattered in the normal brain tissues (100%) and greatly decreased in human gliomas. The expression of NogoB was decreased with the increase of the malignancy in our 36 specimens, being 88.2%，42.9%，and 25.0% in ⅠⅡ,Ⅲ, and Ⅳ grades, respectively (P<0.01)．Conclusion: NogoB is decreases in human glioma at both protein and mRNA level. NogoB participats in the development and progression of bliomas, and its downregulation is closely related to the malignancy of tumors.
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Project Supported:
Supported by the National High Technology Research and Development Program (863) of China（No.2007AA02Z483）; the Second Military Medical University Foundation for Young Scholars (No. 07QN29)
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