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Soluble mucin 1 peptide inhibits proliferation of T lymphoma Jurkat cells and its related mechanisms
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Abstract:
Objective: To investigate the inhibitory effect of mucin 1 (MUC1) peptide on the proliferation of T lymphoma Jurkat cells and the related mechanism. Methods: Jurkat cells were cocultured with MUC1 peptide and effects of MUC1 peptide on Jurkat cell growth was examined by Trypan Blue staining. Cell cycle of Jurkat cells and membrane MUC1 protein expression were determined by flow cytometry. The apoptosis of Jurkat cells was examined by Annexin V/PI double staining. To further analyze whether soluble MUC1 peptide interacts with membrane MUC1 protein, MUC1 protein binding sites on the surface of Jurkat cells was blocked by an antiMUC1 polyclonal antibody. Results: Proliferation of Jurkat cells was inhibited by soluble MUC1 peptide in a dosedependent manner (MUC1 at 10, 20, and 40 μg/ml resulted in inhibitory rates of （32±4）%, （37±2）%, and（46±5）%, respectively ). No apoptosis was observed. Soluble MUC1 peptide induced G0/G1 phase cell cycle arrest of Jurkat cells and membrane MUC1 protein was expressed on the surface of Jurkat cells. The inhibition of Jurkat cells by soluble MUC1 peptide was almost totally reversed by antiMUC1 polyclonal antibodies at the concentrations of 5 μg/ml to 25 μg/ml. Conclusion: It is suggested that soluble MUC1 peptide can inhibit proliferation of Jurkat cells by interacting with membrane MUC1 protein on Jurkat cells and inducing G0/G1 phase cell cycle arrest.
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Project Supported:
Surpported by the Science and Technology Development Program of Jilin Province（No.20080931）
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