Allicin combined with vinorelbine increases both p21 and p27 expression in human gastric cancer cell lines BGC823 and SGC7901

doi:10.3872/j.issn.1007-385X.2009.1.008

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Archive > Volume 16 Issue 1 > 2009,16(1):34-39. DOI:10.3872/j.issn.1007-385X.2009.1.008 Prev Next

Basic Research

Allicin combined with vinorelbine increases both p21 and p27 expression in human gastric cancer cell lines BGC823 and SGC7901

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Abstract:

Objective: To study whether allicin combined with cell cycle specific chemotherapeutic drugs can influence p21，p27 expression in human gastric cancer cell line, so as to discuss the synergistic effect between allicin and cell cycle specific chemotherapeutic drugs and the possible mechanism. Methods： MTT was used to observe the inhibition of gastric cancer cell lines BGC823 and SGC7901 after treatment with vinorelbine(NVB), fluorouracil(5FU) and mitomycin(MMC), and the IC50 was calculated. The change of cell cycle was examined by flow cytometry; SP immunohistochemistry was used to detect the expression of p21 and p27 in BGC823 and SGC7901 cells. Results： Allicin decreased cells in G0/G1 phase and increased cells in G2/M phase in both BGC823 and SGC7901 cells after 24 h. Allicin dose dependently increased expression of p21 and p27 at a serial concentration of 5, 10, 15 and 20 μg/ml. Combination of allicin with NVB increased the expression of both p21 and p27 in both cell lines compared with either allicin or NVB alone（P<001）. Combination of allicin with 5FU or MMC did not further increase the expression of p21 and p27. Conclusion： Combination of allicin with NVB greatly increases expression of p21 and p27 in gastric BGC823 and SGC7901 cells, and subsequently induces cell cycle arrest in G2/M phase.

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