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Tissuespecific CD/5-FC system thermochemotherapy in treatment of a liver metastasis model of colon cancer in nude mice: the safety
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Abstract:
Objective: To study the safety of tissuespecific cytosine deaminase /5fluorocytosine (CD/5FC) system thermochemotherapy in treatment of a liver metastasis model of colon cancer in nude mice. Methods: Liver metastasis model was established by intravenously injection of LOVO cell lines harboring tissuespecific CD gene in 30 nude mice. The mice were then randomly divide into three groups, namely, a control group, a thermochemotherapy group, and a chemotherapy group; the three groups were intraperitoneally injected with sodium saline, 43 ℃ prodrug 5 FC or prodrug 5FC, respectively. After 21 days, the animals were sacrificed and the pathological changes of liver, stomach, lung, pancreas, small intestine, and large intestine were examined by HE staining. RTPCR was used to study the expression of CD gene. Results: The growth of tumor cells in the control group grew actively, and the growth in the 43 ℃ prodrug 5 FC group was greatly inhibited compared with those in the prodrug 5FC and normal saline groups. The normal liver, stomach, lung, pancreas, small intestine and large intestine tissues showed no pathological changes and no CD expression. RTPCR examination revealed stable CD gene expression in the 3 groups. Conclusion: Tissuespecific CD/5FC system thermochemotherapy can improve the specificity of CD gene expression, and reduces the damages to the normal tissues by the thermochemotherapy.
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Surported by the Natural Science Foundation of Fujian Province (No.C0410045)
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