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Inhibitory effect of antisense human telomerase RNA on implanted hepatocellular carcinoma in nude mice
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Abstract:
Objective: To investigate the inhibitory effect of antisense human telomerase RNA (hTR) gene on implanted hepatocellular carcinoma in nude mice. Methods: HepG2 cells were subcutaneously inoculated into BALB/c nude mice at the axilla to establish implanted hepatocellular carcinoma model. The retrovirus plasmid containing antisense telomerse RNA (PLXSNhTRBamHⅠ) was injected into the tumor (0.2 ml every time, 5 times). Retrovirus plasmid containing sense telomerase RNA (PLXSNhTREcoRⅠ) and normal saline were inoculated as control groups. Tumor volume was determined and the inhibitory rate was calculated. Tumor necrosis was observed by histological analysis and cell apoptosis was analyzed by terminal transferase dUTP nick end labeling (TUNEL). Results: Tumor growth in antisense hTR group was significantly inhibited compared with the two control groups. The tumor inhibitory rate (26.78%) of antisense hTR group was significantly higher than that of sense hTR group (1.93%, P＜0.01）). The tumor necrosis area and apoptotic rate in the antisense hTR group were significantly higher than those of the other two groups(P＜0.01）. Conclusion: Antisense hTR can inhibit the growth of implanted hepatocellular carcinoma and promote cell apoptosis.
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Supported by the Medical Science Research Project of Shandong Province (No．CAIDBA3)
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