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Archive > Volume 16 Issue 1 > 2009,16(1):67-70. DOI:10.3872/j.issn.1007-385X.2009.1.015 Prev Next

Basic Research

Effects of sodium phenylbutyrate on invasive ability of human thyroid follicular carcinoma cell line and expression of MMP-9 and TIMP-1

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    Abstract:
    Objective: To investigate the effects of sodium phenylbutyrate (NaPB) on the matrix metalloproteinase9(MMP9) and tissue inhibitor of metalloproteinase1(TIMP1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW1. Methods: CGTHW1 cells were treated with different concentrations of NaPB, then the invasive ability of CGTHW1 cells was assessed using Transwell assay. The expression of MMP9 and TIMP1 was examined by immunocytochemistry staining and RTPCR in CGTHW1 cells. Results: After treatment with NaPB (4 mmol/L) for 72 h, CGTHW1 cells passing the Transwell were significantly reduced\[(29.8±1.77) vs (11.00±2.59)，P<005\]. Immunocytochemistry staining and RTPCR demonstrated that protein and mRNA expression of MMP9 and TIMP1 were significantly downregulated in CGTHW1 cells after NaPB treatment (4, 6 mmol/L) (all P<005).Conclusion: NaPB can inhibit CGTHW1 cell invasion by downregulating MMP9 and TIMP1 expression.

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    Project Supported:
    Surpported by the Secience and Tecnology Program of Jinzhou (No.08B1E28)

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