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Archive > Volume 16 Issue 1 > 2009,16(1):71-74. DOI:10.3872/j.issn.1007-385X.2009.1.016 Prev Next

Basic Research

Effect of AdIL-12 infected mesenchymal stem cells on proliferation of C6 glioma cells

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    Abstract:
    Objective: To study the influence of mesenchymal stem cells (MSC) infected by AdIL12 on the proliferation of C6 glioma cells. Methods: The MSCs were cultured from rat bone marrow and verified by immunohistochemistry and flow cytometry. Recombinant adenovirus vectors harboring IL12 (AdEasyIL12) were infected into MSCs to construct AdIL12MSC containing exogenous IL12. RTPCR and Western Blotting were used to detect IL12 mRNA and protein expression in AdIL12MSC. MTT method was used to detect the effect of AdIL12MSC supernatant on the proliferation of C6 glioma cells. Results: Exogenous IL12 gene was effectively transfected into MSCs by recombinant adenovirus vectors. IL12 was expressed in MSCs at both mRNA and protein levels as detected by RTPCR and Western Blotting. The supernatant of AdIL12MSC significantly inhibited the proliferation of C6 glioma cells compared with MSC supernatant（P<0.05）. Conclusion: MSCs tansfected with IL2 gene (AdIL12MSC) can express IL12 at mRNA and protein levels and inhibit the proliferation of C6 glioma cells.

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    Project Supported:
    Surpported by the Secience and Tecnology Program of Jinzhou (No.08B1E28)

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