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Archive > Volume 16 Issue 2 > 2009,16(2):161-164. DOI:10.3872/j.issn.1007-385X.2009.2.012 Prev Next

Basic Research

Antitumor effects of Apoptin gene against hepatoma cells in vivo and in vitro

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    Abstract:
    Objective:To investigate the antitumor effects of Apoptin against human hepatocellular carcinoma HepG2 cells and implanted H22 tumors in C57BL/6 mice. Methods: C57BL/6 mice were used to establish H22bearing models. pVAX1Apoptin was intratumorally injected and the inhibition of tumor growth was observed. Recombinant plasmid pVAX1Apoptin was transfected into HepG2 cells by Lipofectamine. The expression of Apoptin protein in HepG2 cells was examined by Western blotting. Antitumor effect of pVAX1Apoptin on HepG2 cells was measured by MTT assay, and the apoptosis of HepG2 cells after transfected with pVAX1Apoptin was observed by AO/EB staining.Results:Apoptin gene was effectively expressed in HepG2 cells after transfection with pVAX1Apoptin. pVAX1Apoptin induced apoptosis of HepG2 cells and inhibited the growth of HepG2 cells, with the suppression rate being 69.28% at 48 h after transfection. Intratumoral injection of pVAX1Apoptin significantly inhibited tumor growth, with tumor inhibition rate being 46.71% and mice survival rate being 40% at 39 d after injection. Conclusion: pVAX1Apoptin can inhibit the proliferation of HepG2 cells, and intratumoral injection of pVAX1Apoptin can greatly inhibit tumor growth in vivo.

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    Project Supported:
    Supported by the National High Techology Research and Development Program (863) of China (No. 2007AA021004)

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