Mobile website
Basic Research
Influenza vaccine enhances function of dendritic cells derived from bone marrow of patients with myeloid leukemia
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To determine whether the influenza vaccine can affect the function of dendritic cells（DCs）derived from the bone marrow of patients with myeloid leukemia and the possible mechanism. Methods: The bone marrow (BM) mononuclear cells were obtained from 19 patients with acute myelocytic leukemia (AML) and 8 patients with chronic myeloid leukemia (CML), and were cultured with GMCSF and IL4 for 7 days to obtain immature DCs. Then DCs were stimulated by whole inactivated influenza vaccine (WIV), split influenza vaccine (SIV), or TNFα. After 24 h, phenotypes and karyotypes of these DCs were assessed by FACS and R band karyotype analysis, respectively. The supernatant IL12 levels were measured by ELISA in each group. Cytotoxic activity of CTL induced by differently treated DCs was measured by CCK8 assay.Results: DCs were successfully induced in 15 of the 19 AML patients and all the 8 CML patients. After stimulated with WIV or SIV for 24 h, DCs exhibited enhanced expression of CD83, CD86 and HLADR, and increased secretion of IL12 (all P<0.05). CTL induced by WIV or SIVstimulated DCs specifically killed autologous leukemia cells in vitro (P<0.05). Furthermore, WIVstimulated DCs were more powerful than SIVstimulated DCs in killing target cells (P<0.05). Conclusion: Influenza vaccine can promote the maturation and IL12 secretion of DCs derived from myeloid leukemia patients, and CTL induced by influenza vaccinestimulated DCs has a stronger ability to kill autologous leukemia cells.
Keywords:
Project Supported:
Supported by the Science Research Program of Gansu Province（No.0709TCYA063）
Clc Number:
