Prediction and identification of survivin specific HLA-A2+ CTL restricted high affinity epitope

doi:10.3872/j.issn.1007-385X.2009.3.004

WeChat

Mobile website

Archive > Volume 16 Issue 3 > 2009,16(3):227-232. DOI:10.3872/j.issn.1007-385X.2009.3.004 Prev Next

Basic Research

Prediction and identification of survivin specific HLA-A2+ CTL restricted high affinity epitope

Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials

Abstract:

Objective: To predict and identify survivin specific HLAA2+ CTL restricted epitopes by bioinformatic methods, so as to provide a foundation for survivinbased immunotherapy. Methods: Survivin specific HLAA2+ restricted CTL epotides were predicted by computer supermotif algorithm combined with quantitativemotif algorithm. Candidate epitopes were verified when their scores were higher than 10 and were then artificially synthesized. Affinity of candidate epitope was examined by HLAA2 binding assay combined with flow cytometry using T2 cells (shown as fluorescence index, FI). Stability of candidate epitope was evaluated by HLAA2 dissociation assay combined with flow cytometry (shown as 50% complex dissociation time, DC50). Results: Nine candidate epotides were obtained: 20STFKNWPFL28 (SV2028), 23KNWPFLEGC31 (SV2331), 96LTLGEFLKL104 (SV96104), 6LPPAWQPFL14 (SV614), 33CTPERMAEA41 (SV3341), 46CPTENEPDL54 (SV4654), 130KVRRAIEQL138(SV130138), 37RMAEAGFIH45(SV3745), and 88SVKKQFEEL96 (SV8896). HLAA2 binding assay showed that FI values of SV2028, SV96104, SV130138 and SV2331 epotides were 8.61, 688, 5.89 and 3.81, respectively; those of SV3341, SV614, SV4654, SV3745 and SV8896 epotides were 0.31, -0.29,-04,-0.16 and -0.03, respectively. HLAA2 dissociation assay showed that DC50 values of SV2028, SV96104 and SV130138 epotides were longer than 8 h; that of SV2331 epotide was 46 h; those of SV614, SV3341and SV8896 epotides were all 24 h; those of SV4654, SV3745 epotides were both 02 h. The above results demonstrated that SV2028, SV96104 and SV130138 were high affinity epotides; SV2331 was intermediate affinity epotide; and SV3341, SV614, SV4654, SV3745 and SV8896 were low affinity epotides. Conclusion: Antigen epitope can be quickly and efficiently predicted by supermotif algorithm combined with quantitativemotif algorithm. SV2028, SV96104 and SV130138 epitopes are survivin specific HLAA2+ restricted CTL epotides, which can be used for later research.

Keywords:

Project Supported:

Supported by the Key Science and Technology Development Program of Fujian Province (No.2008I0012)

Clc Number:

More...

More...