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Inhibitory effect of antiATPase F1α antibody against lung adenocarcinoma A549 cells in mice
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Abstract:
Objective:To study the inhibitory effect of a new antiATPase F1α antibody, antihuman angiostatin interacting and tumor metastasis involving protein (HAITMIP) antibody (MAb3D5AB1,GX), against lung adenocarcinoma cell line A549 in mouse tumor model. Methods: The tumorbearing mouse model was established by injecting A549 cells into the right infraaxillary dermis of C57BL/6 mice. Thirtytwo mice were evenly randomized into 4 groups. The control group was untreated; group A, B, and C were treated with different concentrations of GX (125, 250, 500 ng/ml, respectively). The tumor volume, tumor growth delay (TGD) and survival time of mice was observed in all groups. Microvascular density (MVD) of the tumors was determined by immunocytochemistry and apoptosis of tumor cells was examined by TUNEL assay. Results:Human lung adenocarcinoma A549 implanted animal model was successfully established in C57BL/6 mice. Tumor volumes in all GXtreated mice were smaller than that in control mice (P<0.05). TGD in all GXtreated mice was prolonged as the concentration of GX increasing. After treatment with GX, the MVD in tumors was significantly decreased (P<0.05) and survival time was increased (P<0.05) compared with those in control group. TUNEL results revealed that apoptosis rate of tumor cells of GXtreated mice was higher than that in control mice (P<001). Conclusion: GX can inhibit angiogenesis in implanted A549 tumors and promote apoptosis of A549 cells, thus inhibiting tumor growth and prolonging survival of tumorbearing mice.
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Supported by the Medical Science Research “11th FiveYear Plan” of PLA（No.1MA159）
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