Effects of Smad4 overexpression on proliferation of human gastric cancer cells and activation of NF-κB pathway

doi:10.3872/j.issn.1007-385X.2009.3.008

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Archive > Volume 16 Issue 3 > 2009,16(3):248-252. DOI:10.3872/j.issn.1007-385X.2009.3.008 Prev Next

Basic Research

Effects of Smad4 overexpression on proliferation of human gastric cancer cells and activation of NF-κB pathway

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Abstract:

Objective:To construct pEGFPC1Smad4 expression vector and to observe the influence of Smad4 overexpression on the proliferation of human gastric cancer SGC7901 cells and its relationship with nuclear factor kappa B (NFκB). Methods:Recombinant expression vector pEGFPC1Smad4 was constructed and was used to transfect human gastric cancer SGC7901 cells. EGFP expression in transfected cells was detected by fluoroscopy. Smad4 and NFκB expression in transfectant was examined by Western blotting. Effect of Smad4 overexpression on activation of NFκB and proliferation of transfected SGC7901 cells were examined by electrophoretic mobility shift assay (EMSA) and MTT assay, respectively. Results: Expression of EGFP in transfected SGC7901 cells was observed under fluorescence microscope. Smad4 was overexpressed in transfected SGC7901 cells, accompanied by downregulation of NFκB p65 expression in the tranfectants. EMSA and MTT demonstrated that Smad4 overexpression significantly inhibited the activation of NFκB and the proliferation of SGC7901 cells (P<0.05, P<0.01).Conclusion: Smad4 overexpression can greatly inhibit the proliferation of human gastric cancer cells, probably through downregulation of NFκB pathway.

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Supported by the Medicine and Health Scientific Research Program of Zhejiang Province (No. 2008B204)

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