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In vivo antitumor effect of tumor cellderived exosomes combined with BCG
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Abstract:
objective: To study the in vivo antitumor effect of exosomes (Exo) combined with bacillus CalmetteGuérin vaccine（BCG）. Methods:Exo was isolated and purified from culture supernatant of E.G7OVA tumor cells by density gradient centrifugation. Protein components of Exo were detected by Western blotting. Exo, BCG, Exo combined with BCG (Exo+BCG) or PBS were preinjected into mice before injection of E.G7OVA cells, and the antitumor effects were observed in each group. Mouse model bearing E.G7OVA cells was established to examine the immunotherapy effects of Exo with or without BCG. Cytotoxity of spleen CTL was measured by LDH in different groups. Results: Exo derived from E.G7OVA cells contained HSP60, OVA, HSC70 and CD63 as detected by Western blotting. Tumorfree rate at 90 d was significantly higher in Exo+BCG vaccinated mice than those in Exo or BCG vaccinated mice as measured by immunoprotective assay (60% vs 20% or 0%，P<0.01). Immunotherapy assay showed that tumor inhibitory effect in Exo+BCG group was significantly higher than those in Exo or BCG groups (P<0.01). CTL results showed that CTL of Exo+BCG vaccinated mice had significantly enhanced ability to specifically kill target E.G7OVA cells compared with those of Exo and BCG groups (P<0.01). Conclusion: BCG as an immunoadjuvant can significantly enhance the antitumor effect of exosomes in vivo.
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Project Supported:
Supported by the Medicine and Health Research Fund of Zhejiang Province (No.2008B023)
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