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Cytokineinduced killer cells specifically inhibits implanted gastric cancer cells
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Abstract:
Objective:To investigate the inhibitory effect of cytokineinduced killer cells (CIK) against implanted gastric cancer cells. Methods: Gastric cancer SGC7901 cells were subcutaneously injected into the inguina of nude mice to establish gastric cancer model. The tumor bearing mice were randomly divided into CIK group and fibroblasts group, in which mice were subcutaneously injected with fluorescence dye SPDiI labeled CIK and fibroblasts HFLI cells, respectively. Distribution of CIK and HFLI cells in different tissues of gastric cancer bearing mice were observed. Meanwhile, tumor volume was measured after different treatments and tumor inhibitory rate was calculated. Tumor necrosis areas in different groups were observed. Results:SPDiI labeled CIK was mainly located in the gastric cancer tissues 10 d after injection, and was hardly detected at the injection sites, liver, spleen and lung tissues (P<0.01); SPDiI labeled fibroblasts were not found in tumors, liver, spleen and lung tissues, and were mainly located in the injection sites. Volume of implanted tumor in CIK treated mice was significantly smaller than that in the control group (P<0.05), and tumor inhibitory rate of CIK group was 29.82%. The necrosis area score of implanted tumors was significantly higher in CIK group compared with that in the control group (P<0.01). Conclusion: CIK exhibits satisfactory ability to specifically kill implanted gastric cancer.
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Project Supported:
Supported by the Natural Science Foundation of Shandong Province (No.Y2006C22)
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