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Effect of modified cell freezing medium on survival rate and activity of dendritic cells
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Abstract:
Objective:To observe the survival rate and activity of dendritic cells (DCs) to induce the activation of cytokine induced killer cell (CIK) after DCs being preserved in modified cell freezing medium (CFM). Methods:PBMCderived DCs were preserved in three different CFMs at -80 ℃ and -196 ℃, respectively. CFM I was RPMI 1640 containing 10% DMSO, 20% FCS; CFM Ⅱ was Cellbanker (ZENOAQ company, Japan); CFM Ⅲ was modified CFM containing DMSO, hydroxyethylamyle and cell stabilizer. Survival rate of DCs and antitumor activity of DCs activatied CIK (DCCIK) were measured by Trypan blue dye exclusion method and MTT after DCs being preserved in three different CFMs for 30, 60 and 90 d at -80 ℃ or -196 ℃, respectively. Results: Survival rate and activity of DCs preserved in three different CFMs were only slightly reduced as storage period was increased, and there were no significant differences among three CFMs. Conclusion:Modified CFM can substitute traditional and imported Cellbanker CFM in DCs freezing with bright future.
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