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Icariin promotes differentiation and maturation of cord bloodderived dendritic cells
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Abstract:
Objective:To investigate the in vitro effect of icariin (ICA) on the differentiation, maturation and immune activity of DCs derived from cord blood mononuclear cells.Methods: The mononuclear cells were isolated by density gradient centrifugation from cord blood under axenic condition, and were induced by GMCSF and IL4 to differentiate into DCs. The DCs were divided into 3 groups on the fifth day: an ICA group, a TNFα group and a control group. The morphology of DCs was observed under inverted microscope and transmission election microscope. Expression of CD83, CD80 and CD86 on DCs was examined by flow cytometry. IL12 and IFNγ levels in the culture supernatant of DCs were detected by ELISA. The proliferation of T cells stimulated by DCs was determined by MTT assay.Results: Cord blood mononuclear cells derivedDCs had a typical morphological characteristic of mature DCs after stimulation with ICA. Expression of CD1a, CD83, CD80 and CD86 on DCs in ICA group was upregulated compared with that in control group (P<0.05); moreover, CD86 expression on DCs in ICA group was significantly higher than that in TNFα group (P<0.05). IL12 and IFNγ production in ICA group and proliferation of T cells induced by DCs in the ICA group were markedly higher than those in the control group (P<0.05), and were similar to those in the TNFα group.Conclusion: ICA can induce differentiation and maturation of DCs derived from cord blood mononuclear cells, and enhances the immune activity of DCs.
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Project Supported:
Support by the Natural Science Foundation of Hebei Province (No. C2008000952)
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