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Archive > Volume 16 Issue 4 > 2009,16(4):342-346. DOI:10.3872/j.issn.1007-385X.2009.4.005 Prev Next

Basic Research

Inhibitory effects of Ki67 genetargeting peptide nucleic acids linked with dihydrotestosterone on hormoneindependent prostate cancer cells

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    Abstract:
    Objective:To investigate the effects of Ki67targeting peptide nucleic acids (PNAs) linked with dihydrotestosterone (DHT) on the Ki67 expression, proliferation and apoptosis of hormoneindependent prostate cancer cell line PC3. Methods: Ki67targeting PNAs were artificially synthesized and were covalently linked to DHT to yield DHTPNAs. DHTPNAs were then transfected into PC3 cells. Ki67 expression in PC3 cells was examined by RTPCR, immunohistochemistry and Western blotting assay. The proliferation of PC3 cells was examined by CCK8 assay, and the apoptosis of PC3 cells was detected by TUNEL assay. Groups treated with PNAs or DHT served as controls. Results: Both PNAs and DHTPNAs inhibited the expression of Ki67 in PC3 cells, increased the apoptotsis of PC3 cells, and inhibited the proliferation of PC3 cells in a dosedependent manner. The therapeutic effect of DHTPNAs at the same concentration was better than that of PNAs, with 3 μmol/L DHTPNAs (onethird of PNAs dose) reaching the same therapeutic effect of PNAs.Conclusion: DHTPNAs can promote the effects of PNAs in inhibiting Ki67 expression, inducing apoptosis and inhibiting proliferation of PC3 cells.

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    Project Supported:
    Supported by the Medical Science Research Foundation of Guangdong Province (No.A2005805)

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