Effects of AP-2αon invasive growth and ER-β expression of colon cancer cell line

doi:10.3872/j.issn.1007-385X.2009.4.008

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Archive > Volume 16 Issue 4 > 2009,16(4):358-363. DOI:10.3872/j.issn.1007-385X.2009.4.008 Prev Next

Basic Research

Effects of AP-2αon invasive growth and ER-β expression of colon cancer cell line

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Abstract:

Objective:To study the effects of transcription factor activator protein2α (AP2α）on invasive growth and estrogen receptorβ (ERβ) expression in human colon cancer SW620 cells, and to probe into the involved molecular mechanism.Methods: Plasmid pcDNA3.1(＋) AP2α and pcDNA3.1(＋) were transfected into SW620 cells by liposomemediated transfection. The adhesion, invasion and migration abilities of SW620 cells were measured by metrical gel adhesion assay and modified Boyden chamber (Transwell assay). The gene and protein expression levels of AP2α and ERβ in SW620 cells were examined by Realtime PCR, Western blotting and immunofluorescence cytochemistry. The interaction between AP2α DNA and ERβ in SW620 cells was measured by electrophoretic mobility shift assay (EMSA) afterAP2αgene transfection.Results: Overexpression of AP2α markedly reduced the adhesion, invasion and migration abilities of SW620 cells (all P<0.05); meanwhile, the mRNA and protein levels of ERβ in SW620 cells were also significantly enhanced (P<0.05). EMSA results showed that AP2α specifically bound to promoter region of ERβ gene in SW620 cells after transfection of pcDNA3.1(+)AP2αplasmid. Conclusion: Overexpression of AP2α can inhibit the adhesion, invasion and migration abilities of SW620 cells, which is probably related to ERβ expression in SW620 cells directly induced by interaction between AP2α and promoter region of ERβ.

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Supported by the Science and Technology Development Program of Shanxi Province（No.2006031087）

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