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Inhibitory effect of gefitinib combined with DNA vaccine targeting EGFR against mouse lung cancer Lewis cells
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Abstract:
Objective:To observe the inhibitory effect of gefitinib combined with DNA vaccine targeting EGFR against implanted Lewis tumors in mice.Methods: Chicken EGFR L2 domain and rabbit IgG Fc domain fusion pVAX1/cEGFRrFc DNA vaccine was injected into mice and the titer of antiEGFR in serum was determined by ELISA. The growth of Lewis cells was measured by MTT. Lewis lung cancer mouse models were established and were randomly divided into vaccine, gefitinib, gefitinib+vaccine, and control groups. The tumor volume and weight and survival of mice were examined in different groups.Results:The titer of antiEGFR in mice vaccinated with pVAX1/cEGFRrFc plasmid was 1∶1 000. The proliferation of Lewis cells in antiEGFR combined with gefitinib was significantly inhibited compared with those in antiEGFR and gefitinib groups (P<0.05). In vivo results showed that the growth of implanted Lewis tumors in vaccine+gefitinib group was greatly inhibited, and the survival rate of Lewisbearing mice was significalty increased (P<001). Conclusion：DNA vaccine targeting EGFR has a synergistic effect with gefitinib in antitumor activity, and this DNA vaccine can increase the efficiency of gefitinib.
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Project Supported:
Supported by the Key Project of Science and Technology Commission of Shanghai Municipality （No.03DZ19236); the Research Project of Shanghai Pneumology Hospital (No.200612)
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