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Impact of survivintargeted RNAi on growth and apoptosis of implanted human cervical carcinoma in mice
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Abstract:
Objective: To observe the effect of RNAi targeting survivin gene (RNAisurvivin) on the growth and apoptosis of implanted human cervical carcinoma in mice. Methods: Nude mice were randomly divided into 4 groups, and the human cervical carcinoma HeLa cells transfected with pSilencer2.1s2, pSilencer2.1NC, pSilencer2.1U6 neo or not transfected (the cells being HeLas2, HeLaNC, HeLaU6 neo and Hela, respectively) were subcutaneously inoculated into flank tissues of mice to establish cervical carcinoma mouse models. The influence of survivinRNAi on the implanted tumor growth was observed. Expression of survivin protein and microvessel density (MVD) in implanted cervical carcinoma tissues were examined by immunohistochemistry. Apoptosis of implanted cervical carcinoma cells was observed by HE staining and TUNEL. Results: Implanted human cervical carcinoma mouse model was successively established. The tumor weight in HeLas2 group was markedly lower than those in HeLaNC, HeLaU6 neo and Hela groups（P<0.05）. The tumor inhibitory rate in HeLas2 group was 67.9%. Survivin expression and MVD value in tumor tissues of HeLas2 group were significantly decreased as determined by immunohistochemistry（all P<0.05）. Apoptosis of implanted cervical carcinoma cells in HeLas2 group was significantly increased compared with those in other 3 groups（all P<0.05）, with AI being (22.73±1.37)%. Conclusion:RNAisurvivin inhibits the growth and promote apoptosis of implanted human cervical carcinoma tumors by downregulating survivin protein expression and MVD in tumor tissues.
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Project Supported:
Surpported by the Natural Science Foundation of Shaanxi Province (No.2007c251)
Clc Number:
