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Archive > Volume 16 Issue 4 > 2009,16(4):379-382. DOI:10.3872/j.issn.1007-385X.2009.4.012 Prev Next

Basic Research

IL-2 and IL-15 upregulate NKG2D expression and enhance cytotoxicity of editedNK cells against nasopharyngeal carcinoma cells

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    Abstract:
    Objective:To study the effects of IL2 and IL15 on the expression of NKG2D and the cytotoxicity of editedNK cells against human nasopharyngeal carcinoma cell line CNE2. Methods: NK cells were purified by antiCD56 MACS and were divided into four groups: noneditedNK cells group (NK cells treated with 100 U/ml IL2), editedNK cells group (NK cells cocultured with CNE2 cells at a ratio of 10∶1 and then treated with 100 U/ml IL2), editedNK cells retreated with 1 000 U/ml IL2 group, and editedNK cells retreated with 10 ng/ml IL15 group. Expression of NKG2D in each group was determined by FACS 24 h later. Cytotoxicity of NK cells against CNE2 cells (NK∶CNE2 being 20∶1) was measured by LDH releasing assay.Results: The expression of NKG2D in noneditedNK cells, editedNK cells, editedNK cells retreated with IL2, and editedNK cells retreated with IL15 were (97.63±0.83)%, (53.50±1.25)%, (94.47±1.00)%, and (98.07±0.21)%, respectively. The expression of NKG2D on editedNK cells retreated with IL2 or IL15 was significantly increased than that on editedNK cells (P<0.01). The cytotoxicity of noneditedNK cells, editedNK cells, editedNK cells retreated with IL2, and editedNK cells retreated with IL15 against CNE2 cells were (35.90±3.27)%, (4.70±2.30)%, (31.70 ±3.56)% and (40.18±2.94)%, respectively. The cytotoxicity of editedNK cells was significantly enhanced after retreated with IL2 or IL15 (P<0.01), with those retreated with IL15 being stronger than those retreated with IL2.Conclusion: High dose IL2 and IL15 can upregulate the expression of NKG2D on editedNK cells and restore their cytotoxicity against CNE2 cells, with the efficacy of IL15 stronger than that of IL2.

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    Project Supported:
    Supported by the National Natural Science Foundation of China (No.30471663)

    Clc Number:
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