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Knockdown of OPN by OPNRNAi inhibits growth and invasiveness of U87 glioma cells
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Abstract:
Objective:To study the effect of siRNA targeting osteopontin（OPNRNAi）on the proliferation and invasiveness of U87 glioma cells and the possible mechanism. Methods: OPNRNAi was synthesized according to the gene sequence of OPN protein and was transfected into U87 cells. The proliferation of U87 cells was examined by MTT; matrix metalloprotease (MMP) 2 and MMP9 expression were detected by Western blotting assay; transwell assay and gelatinzymogram were used to detect the invasion ability of U87 cells and gelatinase acitivity of MMP2 and MMP9, respectively.Results:Synthesized OPNRNAi effectively inhibited the expression of OPN in U87 cells (P<0.05). OPNRNAi also significantly decreased the expression of MMP2 and MMP9 in U87 cells (P<0.05) and the gelation activity of MMP2 and MMP9 (P<0.01), and inhibited the proliferation and invasivenss of U87 cells（all P<0.05）. Conclusion: Knockdown of OPN with OPNRNAi can inhibit the proliferation and invasiveness of U87 cells, which is probably related to the decreased expression of MMP2 and MMP9 genes and their gelatinase acitivities.
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Project Supported:
Supported by the National Natural Science Foundation of China (No. 30672165); the Medical Talent Project of Jiangsu Province (No.RC2007061)
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