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Therapeutic effect of local implantation with temozolomide/PLGA microsphere in treatment of rat C6 glioma in vivo
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Abstract:
Objective:To study the therapeutic effect of local implantation with temozolomide/PLGA microsphere (TMMS) on rat C6 glioma in vivo.Methods: C6 glioma cells were implanted stereotaxically to rat caudate nucleus of left cerebrum to establish C6 gliomarat model. C6 gliomarats were treated with oral temozolomide or with TMMS (implanted in cerebral tumor foci). The general manifestation, survival time, tumor size and pathological changes were observed in each group. The expression of proliferation cell nuclear antigen (PCNA) in glioma tissues was examined by immunohistochemistry method. Apoptosis of glioma cells was measured by TUNEL.Results: The survival period of C6 gliomarats in TMMS group was longer than those in the sham group, blank microsphere group and oral Temozolomide group (being \[31.2±6.21\] vs \[20.7±4.83\], \[19.2±6.23\] and \[24.7±6.31\] d, respectively, P<0.05 or P<0.01). MRI results demonstrated that the volume of glioma in interstitial TMMS group was smaller than those in the sham group, blank microsphere group and oral Temozolomide group (being \[28.8±6.41\] vs \[56.4±6.92\], \[58.2±5.36\] and \[46.7±7.28\] mm3, respectively, P<0.05 or P<0.01). PCNA expression in glioma tissues of TMMS group was significantly lower compared with those in the sham group, blank microshere group and oral Temozolomide group (being \[202±433\]% vs \[63.2±5.91\]%, \[62.1±7.88\]% and \[41.7±6.71\]%, respectively, P<0.01). Apoptosis rate of glioma cells in TMMS group was markedly higher compared with those in the sham group, blank microshere group and oral Temozolomide group (being \[32.31±3.17\]% vs \[8.63±1.52\]%, \[9.25±2.31\]% and \[16.14±3.42\]%, respectively, P<001).Conclusion: Interstitial TMMS therapy effectively inhibits proliferation and induces apoptosis of glioma cells in mice, and it has a potential in clinic tumor therapy.
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Project Supported:
Supported by the Key Scientific and Technological Project of Shanghai Scientific and Technological Commission（No.08411953600)
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