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Troglitazone inhibits growth of pituitary adenoma GH3 cells and the involved mechanisms
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Abstract:
Objective:To examine the effects of troglitazone (TGZ), agonist of peroxisome proliferatoractivated receptorγ (PPARγ), on the growth and growth hormone (GH) secretion of pituitary adenoma cells, and to expolre the possible mechanism. Methods: The inhibitory effect of TGZ on the proliferation and GH secretion of rat pituitary adenoma cell line GH3 was detected by MTT assay and ELISA. Furthermore, apoptosis, cell cycle as well as caspase3, Bcl2 and Bax expression of GH3 cells were examined by transmission electron microscopy, flow cytometry and Western blotting, respectively. Results: TGZ dose and timedependently inhibited the proliferation and GH secretion of GH3 cells. GH3 cells treated with TGZ had a typical morphological characteristic of apoptosis. CH3 cell number in G1 phase was increased and cell number in G2, S phases was significantly decreased after treatment with TGZ. The expression of caspase3 and Bax in CH3 cells was significantly increased and Bcl2 expression was markedly decreased in a dosedependent manner after treatment with TGZ. Conclusion: PPARγ agonist inhibits the growth and GH secretion of pituitary adenoma cells through inducing apoptosis and cell cycle arresting.
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Project Supported:
Surpported by the Natural Science Foundation of Hainan(No.30852)
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