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XIAP targeting siRNA inhibits proliferation of colorectal cancer cells in vitro and in vivo
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Abstract:
Objective:To investigate the effect of XIAP (Xlinked inhibitor of apoptosis protein)targeting siRNA on the proliferation of LoVo colorectal cancer cells in vitro and in vivo. Methods: The XIAPtargeting siRNA pSil2.1shXIAP1 and pSil2.1shXIAP2 eukaryotic vectors were constructed and were transfected into LoVo cells using Lipofectamine; the stable transfectants LoVoshXIAP2 were selected by G418. The expressions of XIAP mRNA and protein were determined by RTPCR and Western blotting. The proliferation of LoVo cells was determined by MTT and colony formation assay. Cell apoptosis was examined by FCM. The influence of XIAP knockdown on the proliferation of LoVo cells in vivo was observed in LoVobearing nude mice. Results:The expressions of XIAP mRNA and protein in LoVoshXIAP2 cells were significantly downregulated in LoVoshXIAP2 cells. Compared with untransfected LoVo cells, the proliferation and colony formation abilities of LoVoshXIAP2 cells were significantly inhibited (P<0.05); the apoptosis rate of LoVoshXIAP2 cells was significantly increased (P<0.05). The expression of XIAP protein in LoVoshXIAP2 implanted tumors was downregulated and the growth of tumors was significantly inhibited (all P<0.05). Conclusion: pSil2.1shXIAP2 plasmid can downregulate the expression of XIAP in LoVo cells and inhibit proliferation of LoVo cells in vitro and in vivo, making XIAPtargeting siRNA a potential new agent in immune therapy of colorectal cancer.
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