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Inhibitory effect of deuteriumdepleted water on proliferation of lung carcinoma cells and the possible mechanism
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Abstract:
Objective:To explore the in vitro and in vivo inhibitory effects of deuteriumdepleted water (DDW) on the proliferation of human lung carcinoma cells, and to explore the possible mechanism. Methods: The inhibitory effect of DDW on the proliferation of human lung carcinoma A549 cells and human embryonic lung fibroblast HLF1 cells was examined by MTT assay; apoptosis of A549 cells was examined by TUNEL; and cell cycle was analyzed by flow cytometry. Mouse model of lung carcinoma was established by inoculating human lung carcinoma H460 cells into BALB/c nude mice, and the growth of implanted tumors was observed after DDW treatment for 60 d. Results: Compared with control group, A549 cells treated with 0.0025％, 0.0050％ or 0.0105％ DDW showed significantly decreased proliferation 10 h after treatment (P<0.01). Then the inhibitory effects of DDW gradually disappeared, but appeared 48 h later again, with the inhibitory effects at 72 h being significant (P<0.05). DDW at the same dosages showed no inhibition on the proliferation of HLF1 cells (P>0.05). TUNEL assay verified the apoptosis of DDWtreated A549 cells, and the apoptosis rate of DDWtreated A549 cells was significantly higher than that of control group（\[45.30±4.21\]% vs \[22.25±030\]%, P<0.01). Cells in S phase were significantly increased in DDWtreated A549 cells compared with those in the control group (P<0.05). The life quality of H460 cellinoculated nude mice treated with DDW was greatly improved, with the tumor inhibition rate being 30.08%. Conclusion: DDW can inhibit the proliferation of lung cancer cells within a certain range of dosage and in a fluctuating pattern; its mechanism might be associated with induction of apoptosis and cell cycle arrest in S phase of tumor cells.
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Surpported by the Postdoctor Foundation of Shanghai JiaoTong University
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