Mobile website
Basic Research
Curcumine inhibits proliferation of breast cancer MCF-7 cells and the related oxidative stress mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the inhibitory effect of curcumine on the proliferation of breast cancer MCF7 cells and the related oxidative stress mechanism. Methods: Human breast cancer MCF7 cells were treated with different concentrations of curcumine (540 μmol/L), then the proliferation of MCF7 cells was examined by MTT assay after 6, 12, 24 and 48 h; the apoptosis rate of MCF7 cells was detected by flow cytometry (FCM); the levels of reactive oxygen species (ROS) in MCF7 cells were detected by DCFHDA staining; and the activities of superoxide dismutase (SOD) and catalase (CAT) in MCF7 cells were detected by xanthine oxidase assay and visible spectrophotometer, respectively. Results: Low level curcumine (5, 10 μmol/L) inhibited the proliferation of MCF7 cells (P<0.01) without inducing apoptosis; ROS levels in MCF7 cells were firstly increased and then declined gradually; meanwhile, SOD and CAT activities decreased initially and then gradually increased. High level curcumine (20, 40 μmol/L) significantly inhibited the proliferation of MCF7 cells and induced evident apoptosis (P<0.01), and ROS levels in MCF7 cells were elevated instantly and maintained for a long time after treatment; at the same time, SOD and CAT activities were significantly decreased (P<0.01). Conclusion: Curcumine has a double role of antioxidant and oxidation, and it functions in a dosedependent manner. Different concentrations of curcumine can inhibit the proliferation of tumor cells through regulating oxidative statuses in cells.
Keywords:
Project Supported:
Supported by the Scientific Research Project of Jiangsu Health Ministry（No.H200817）
Clc Number:
