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Brucea javanica oil emulsion inhibits peoliferation of cervical cancer SiHa cells
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Abstract:
Objective:To investigate the inhibitory effect of Brucea javanica oil emulsion on cervical cancer SiHa cells in vitro and the related mechanisms. Methods: SiHa cells were treated with Brucea javanica oil emulsion（2.5, 5.0, 10, 20, 40, and 80 μg/ml）for 24, 48, and 72 h. The inhibitory effect of Brucea javanica oil emulsion on the proliferation of SiHa cells was examined by MTT; the ultrastructure of SiHa cells was observed by transmission electron microscope (TEM); the DNA fragment of SiHa cells was detected by agarose gel electrophoresis; and the apoptosis of SiHa cells was examined by flow cytometry through Annexin V and PI staining. Results:MTT results showed that Brucea javanica oil emulsion significantly inhibited the proliferation of SiHa cells in a dose and timedependent manner. The inhibitory rate of SiHa cells reached 92.43% after treatment with 80 μg/ml Brucea javanica oil emulsion for 72 h. Apoptotic bodies and a characteristic apoptosis DNA ladder of SiHa cells were observed after treatment with 20 μg/ml Brucea javanica oil emulsion. Flow cytometry results demonstrated that the apoptosis rates of SiHa cells were (8.02±241)%, (51.60±7.67)%, and (77.22±580)% 48 h after treatment with 10, 20, and 40 μg/ml Brucea javanica oil emulsion for 48 h, respectively. Conclusion: Brucea javanica oil emulsion can inhibit the proliferation of SiHa cells, probably by inducing apoptosis of SiHa cells.
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Project Supported:
Supported by the National Natural Science Foundation of China (No.30670916); the Science and Technology Program of Liaoning Province (No. 200722500522)
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