Mobile website
Clinical Research
DNA polymerase iota expression in human lung cancer and its correlation with clinicopathological and prognosis of cancer
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the expression of DNA polymerase iota (Polι) in human lung cancer tissues, adjacent lung cancer tissues, and normal lung tissues, so as to analyze the role of Polι gene in the development and progression of lung cancer. Methods: Samples were obtained from lung cancer patients who were surgically treated during Aug. 2003 to Jan. 2001 in Qilu Hospital of Shandong University. Immunohistochemisty and RTPCR were applied to detect Polι expression in 60 lung cancer specimens, 30 adjacent lung cancer specimens and 30 normal tissue specimens. Data were analyzed with PSS13.0 software. Results: The positive rates of Polι in lung cancer, adjacent lung cancer tissues and normal tissues were 54.8%, 33.3% and 10.0%, respectively. Polι expression levels were similar in different types of lung cancer (P>005). Polι expression in lung cancer tissues was decreased with the increase of differentiation grade of lung cancer (P<0.05), with the expression in grade Ⅲ and Ⅳ of lung cancer significantly higher than those in grade Ⅰ and Ⅱ(P<0.05). Polι expression in patients who survived less than 3 years was markedly higher than in those survived longer than 3 years (P<005); and smoking patients had a markedly higher expression than nonsmokers (P<0.05). Conclusion: High expression of Polι plays an important role in development and progression of lung cancer, and it may be used in evaluating the biological characteristics and prognosis of lung cancer.
Keywords:
Project Supported:
Supported by the National Natural Science Foundation of China (No.30571844)
Clc Number:
