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In vivo fluorescence image analysis system in assessing efficacies of pVAX1 Ag85A and pVAX1 Ag85B DNA vaccines in treatment of bladder cancer cell implanted tumors in mice
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Abstract:
Objective:To use in vivo fluorescence image analysis system for evaluating the efficacies of pVAX1Ag85A and pVAX1Ag85B DNA vaccines in treatment of bladder cancer cellimplanted tumors in mice. :Methods: :Discosomasp red fluorescent protein (DsRed) stably transfected bladder cancer BTT cell line (BTTDsRed) was established and BTTDsRed cellimplanted mouse model was constructed. Six days later, 24 BTTDsRedbearing mice were randomly divided into pVAX1Ag85A DNA vaccine group, pVAX1Ag85B DNA vaccine group, and saline group through injecting the pVAX1Ag85A, pVAX1Ag85B, and saline into the right hind limbs of mice, respectively. The growth and metastasis of implanted BTTDsRed tumors were examined by in vivo fluorescence image analysis system. :Results: :BTT cell line stably transfected with DsRed (BTTDsRed) was successfully established. Fluorescence visible mouse model was successfully established by inoculating BTTDsRed cells into hind limbs of mice. After treatment with pVAX1Ag85A or pVAX1Ag85B for 2 weeks, the in vivo tumor fluorescence intensity in pVAX1Ag85B group was significantly lower than that in the saline group (P<0.05). After 3 weeks, tumor fluorescence intensities in both pVAX1Ag85A and pVAX1Ag85B groups were significantly lower than that in the saline group (P<0.01). But the efficacies of pVAX1Ag85A and pVAX1Ag85B groups were similar (P>0.05). The distant lymphatic metastasis rate in pVAX1Ag85B group was significantly lower than those in the saline (25.0% vs 87.5%) and pVAX1Ag85A groups (25.0% vs 62.5%) (P＜0.05). :Conclusion:: In vivo fluorescence image analysis system can dynamically, sensitively and visually evaluate the antitumor effects of DNA vaccines against bladder cancer cellimplanted tumors. Both pVAX1Ag85A and pVAX1Ag85B DNA vaccines have antitumor effects for bladder cancers.
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Project Supported:
Supported by the Scientific and Technological Project of Shanxi Province (No. 20090321022)
Clc Number:
