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Rapamycin inhibits growth and metastasis of gallbladder cancer GBC-SD cells
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Abstract:
Objective:To investigate the effect of rapamycin on cell growth and migration of gallbladder cancer GBCSD cells, and to discuss its potential in clinical therapy of gallbladder cancer. :Methods: :Proliferation of GBCSD cells treated with different concentrations of rapamycin （12.5, 25, and 50 mmol/L）was examined by MTT assay. Cell cycle distribution and apoptosis of GBCSD cells treated with different concentrations of rapamycin were determined by flow cytometry. Migration ability of GBCSD cells was assessed by Transwell assay. The expression of mTOR (mammalian target of rapamycin) and its phosphorylation in GBCSD cells were examined by Western blotting assay. :Results: :Rapamycin significantly inhibited the phosphorylation of mTOR, but had no influence on the expression of mTOR in GBCSD cells. Rapamycin significantly inhibited the growth of GBCSD cells in a dosedependent manner (P<0.01). Rapamycin induced apoptosis of GBCSD cells and arrested them at the G1/S phase. Furthermore, rapamycin also significantly suppressed migration of GBCSD cells as showed by Transwell assay (P<0.01). :Conclusion:: Rapamycin can remarkably inhibit the growth and migration of gallbladder cancer cells, probably by inhibition of pmTOR pathway, induction of apoptosis and cell cycle arrest of gallbladder cancer cells.
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