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Preparation of PEI-RGD/125I(αV)ASODN and its inhibitory effect on invasive ability of HepG2 cells
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Abstract:
Objective:To study the effects of 125I(αV)ASODN on the in vitro invasive ability of heptocellular carcinoma cell line（HepG2）through PEIRGDmediated receptor process. :Methods: :Intergrin αVspecific antisense oligonucleotide was labeled with 125I, and PEIRGD/125I(αV)ASODN complex was prepared by combining 125I(αV)ASODN with polyethyleneimine derivative PEIRGD. PEIRGD/125I(αV)ASODN complex was transferred into HepG2 cells through the receptormediated process. The effect of PEIRGD/125I(αV)ASODN complex on the invasive ability of HepG2 cells was examined by Boyden chamber invasive assay. :Results: :(1) The labeling yield and radiochemical purity of 125I(αV)ASODN were（73.78±4.09）% and（96.68±1.38）%, respectively, and the labeled compound had a good stability in vitro after 48 h at 37 ℃; (2) The ability of HepG2 cells to uptake PEIRGD/125I(αV)ASODN reached its peek（\[1277±0.85\]%) when PEIRGD/125I(αV)ASODN was at 4 μl/2 μg（\[12.77±0.85\]%）, and then gredually decreased thereafter. So the dosage of PEIRGD/125I(αV)ASODN for the following experiment was chosen as 2 μl/1 μg; (3) The invasive capacity of HepG2 cells was significantly reduced in PEIRGD/125I(αV)ASODN group compared with those in other experiment and control groups (P<0.01). :Conclusion:: 125I(αV) ASODN mediated by PEIRGD can effectively inhibit the invasive capacity of HepG2 cells.
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Supported by the National Natural Science Foundation of China (No.30300092)
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