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E1A inhibits growth and increases radiosensitivity of tumors in nude mice implanted with nasopharygeal carcinoma cells
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Abstract:
Objective:To study the inhibitory effect of :E1A: gene on the growth of tumors in nude mice implanted with nasopharygeal carcinoma CNE2 cells and its promotion effect on the radiosensitivity of CNE2implanted tumors, and to investigate the related mechanism. :Methods: ::E1A: gene was transfected into CNE2 cells using adenovirus system, and stable :E1A: positive clones were established. The inhibitory effect of :E1A: on tumor formationability of CNE2 cells was observed in nude mice. The efficacy of :E1A: gene therapy with or without radiotherapy against CNE2 cellimplanted tumors was evaluated. The effect of :E1A: gene therapy on the expression of :P53: was detected by RTPCR. :Results: :CNE2 cells stably transfected with :E1A: gene (CNE2AdE1A）were successfully established. The tumor formation time was later and tumor size was smaller in CNE2AdE1A cellimplanted mice compared with those in CNE2 cell and CNE2Adβgal cellimplanted mice (CNE2 cells stably transfected with Adβgal). Radiotherapy, :E1A: gene therapy and :E1A: gene+radiotherapy all suppressed the growth of implanted tumors, with the tumor suppression rates being (60.32±5.34)%, (7053±6.12)%, and (97.15±4.87)%, respectively. :E1A: gene therapy significantly increased the expression of :P53 : gene in tumor tissues. :Conclusion: ::E1A: can inhibit the growth of tumors in mice implanted with nasopharygeal carcinoma cells, and enhance its sensitivity to radiotherapy, which may be related to the increased expression of :P53: gene in tumor tissues.
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Project Supported:
Supported by the National Natural Science Foundation of China (No. 30400118)
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