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Effect of EGFRtargeted interference RNA on apoptosis of multidrugresistant ovarian cancer cells
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Abstract:
Objective:To investigate the effect of RNAmediated interference EGFR (epidermal growth factor receptor) expression on the apoptosis of multidrugresisitant ovarian cancer cell line SKOV3/DDP. :Methods: :Small hairpin RNA (shRNA) targeting EGFR was synthesized and recombinant plasmid containing pEGFRshRNA was constructed. pEGFRshRNA was tansfected into SKOV3/DDP cells by liposome system, untransfected cells and SKOV3/DDP cells tansfected with nonspecificshRNA (CtrlshRNA) were used as control. Expression of EGFR mRNA and protein in SKOV3/DDP cells was examined by RTPCR and immunocytochemistry after transfection, respectively. The apopototic rates and cell cycles of SKOV3/DDP cells were detected by flow cytometry. :Results: :Compared with CtrlshRNAtransfected cells, the expression of EGFR mRNA and protein in pEGFRshRNAtransfected SKOV3/DDP cells was significantly inhibited. Flow cytometry results showed that cell cycle distribution in pEGFRshRNAtransfected SKOV3/DDP cells was dramatically changed, and the apoptosis rate was significantly increased after further treatment with cisplatin for 24 h. :Conclusion: :EGFRtargeted interference RNA can inhibit the expression of EGFR in SKOV3/DDP cells, thereby regulating the cell cycle and increasing apoptosis of multidrugresistant SKOV3/DDP cells.
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