Mobile website
Monographic Report
Inhibitory effect of cochinchina momordica seed ethanol extract on proliferation of melanoma B16 cells and its possible meachanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the effect of cochinchina momordica seed ethanol extract (CMSEE) on the proliferation of melanoma B16 cells and the underlying mechanism. Methods:MTT and clone formation assay were used to assess the effect of CMSEE on the growth of B16 cells. Morphological changes of B16 cells were observed under phasecontrast microscope and Giemsa staining. Cell cycle and apoptosis rate were examined by flow cytometry (FCM). The effect of CMSEE on melanin production and tyrosinase activity of B16 cells was assessed by colorimetry. The effect of CMSEE on the expression of 〖STBX〗Cmyc, P38, and Tyr〖STBZ〗 genes was examined by RTPCR. Results:CMSEE (10100 mg/L) inhibited the proliferation of B16 cell in a doseand timedependent manner. After treatment with 1040 mg/L CMSEE, B16 cells showed typical differentiation morphology, and melanin production and tyrosinase activity were increased. B16 cells treated with 100 mg/L CMSEE showed apoptotic morphology, decreased melanin production and tyrosinase activity. B16 cell number in G0/G1 phase was significantly increased (P<0.01); Cmyc mRNA expression was downregulated, and P38, Try mRNA expression was upregulated in B16 cells after treatment with 1040 mg/L CMSEE. Conclusion:CMSEE can markedly inhibit the proliferation of melanoma B16 cells, which is related to induction of differentiation and promotion of apoptosis of B16 cells.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30371753)
Clc Number:
