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Archive > Volume 17 Issue 1 > 2010,17(1):36-39. DOI:10.3872/j.issn.1007-385X.2010.1.007 Prev Next

Monographic Report

pEGFC1-IGFBP7 induces apoptosis of malignant melanoma cell line SK-MEL-28

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    Abstract:
    Objective:To construct the insulinlike growth factor binding protein 7 (IGFBP7) expression plasmid (pEGFC1IGFBP7) and to investigate the effect of IGFBP7 on the apoptosis of SKMEL28 (human malignant melanoma cell line) cells. Methods: The pEGFC1IGFBP7 plasmid was constructed; pEGFC1IGFBP7 and empty plasmids were transfected into SKMEL28 cells separately. The transfection efficiency was observed under fluorescence microscope. Apoptosis of SKMEL28 cells after transfection was detected by AnnexinFITC/PI staining. Results:The pEGFC1IGFBP7 plasmid was successfully constructed and was effectively transfected into SKMEL28 cells by Effectene reagent, with the transfection rate being 61%. The results of flow cytometry showed that pEGFC1IGFBP7 significantly induced apoptosis of SKMEL28 cells, with the apoptotic rates of pEGFC1IGFBP7, empty vector, and nontransfected plasmid groups being (28.4±2.57)%, (5.8±0.44)%, and (6.4±0.71)% 24 h after transfection, respectively (F=406.138, P<005). Conclusion:pEGFC1IGFBP7 can effectively induce apoptosis of malignant melanoma SKMEL28 cells, which provides an experimental basis for IGFBP7 genebased therapy of malignant melanoma.

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    Project Supported:
    Project supported by New Teacher Education Program of Education Ministry of China (No.20070487140)

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