In vitro and in vivo cytotoxicity effects of co-cultured DC CIK cells combined with sorafenib against hepatocellular carcinoma

doi:10.3872/j.issn.1007-385X.2010.1.009

WeChat

Mobile website

Archive > Volume 17 Issue 1 > 2010,17(1):46-50. DOI:10.3872/j.issn.1007-385X.2010.1.009 Prev Next

Basic Research

In vitro and in vivo cytotoxicity effects of co-cultured DC CIK cells combined with sorafenib against hepatocellular carcinoma

Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials

Abstract:

Objective:To investigate the in vitro and in vivo inhibitory effects of DC (dendritic cell)CIK (cytokineinduced killer cell) cocultured cells combined with sorafenib against hepatocellular carcinoma cell line BEL7402. Methods: DC and CIK cells were generated in vitro by stimulating human peripheral blood mononuclear cells with different cytokines, and then they were cocultured. The cytotoxicity of DCCIK cocultured cells (DCCIK) combined with sorafenib against BEL7402 cells was determined by CCK8 kit. The apoptosis of BEL7402 cells was measured by Annexin VFITC Kit. BEL7402implanted tumor model was established by subcutaneous injection in nude mouse. Tumorbearing mice were divided into normal saline control group, sorafenib group, DCCIK group and DCCIK+sorafenib group. The inhibitory effects were observed in different groups. Results: The cytotoxicity rate of BEL7402 cells in DCCIK+sorafenib group was significantly higher than those in the other two groups, with cytotoxicity rate in DCCIK+sorafenib group being (75.24±1.91)%, which was 1.8 times that in DCCIK group and 2.1 times that in sorafenib group (P<0.01). The apoptosis rate of BEL7402 cells in DCCIK+sorafenib group was significantly higher than those in the sorafenib and DCCIK groups, with the apoptosis rate in DCCIK+sorafenib group being (78.32±2.54)% (P<0.05). The volume of tumor in the combination group was significantly smaller than those in the other groups (P<0.05). In vivo results showed that DCCIK+sorafenib treatment significantly inhibited the growth of BEL7402implanted tumors, and the inhibitory rate was (83.37 ±0.16)%, which was significantly higher than those of the other groups (P<0.01). Conclusion：DCCIK cocultured cells combined with sorafenib can inhibit the growth of hepatocellular carcinoma cell line BEL7402 in vitro and in vivo. Molecular targeting therapy combined with immunotherapy may be a new way for the comprehensive treatment of hepatocellular carcinoma.

Keywords:

Project Supported:

Project supported by the Key Science and Technology Development Program of Liaoning Province (No.20082250085)

Clc Number:

More...

More...