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Grim-19 overepression induces apoptosis of colon cancer SW480 cells
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Abstract:
Objective: To investigate the effect of retinoidinterferoninduced mortality (GRIM-19) gene on the apoptosis of colon cancer. Methods:A GRIM19 eukaryotic expression vector (pCMV-Flag-GRIM-19) was constructed and transfected into SW480 cells. Expressions of GRIM-19 and apoptosisrelated proteins were detected by Western blotting analysis. Apoptosis of SW480 cells was measured by AnnexinV/PI assay and mitochondrial membrane potential JC-1 staining. Results:The GRIM19 eukaryotic expression vector pCMVFlagGRIM-19 was successfully constructed. Expression of GRIM19 in SW480 cells was upregulated and that of apoptosisrelated protein Bclxl was downregulated after transfection with pCMV-Flag-GRIM-19. Apoptosis rate was (7.7±1.39)% in SW480 cells transfected with pCMV-Flag empty vector and (15.0 ± 2.52)% in pCMV-Flag-GRIM-19 transfected cells (P<0.05). Mitochondrial membrane potential was decreased in (7.5±2.09)% of pCMVFlag transfected cells and (17.5±3.07)% of pCMV-Flag-GRIM-19 transfected cells (P<0.05). Conclusion:In vitro GRIM-19 transfection can effectively induce apoptosis of colon cancer SW480 cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30471673, 30872472, 30800569), and the Major State Basic Research Development Program (973) of China (No. 2009CB521802)
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