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Screening of differentially expressed genes in osteosarcoma cell lines with various metastatic potentialities
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Abstract:
Objective:To investigate the differentially expressed genes in osteosarcoma cell lines with various metastatic potentialities, and to screen for new candidate genes related to metastasis of osteosarcomas. Methods:The total RNAs of a lowly metastatic and a highly metastatic osteosarcoma cell lines (M6 and M8) were extracted. Differentially expressed genes in the two osteosarcoma cell lines were studied by cDNA microarray. The hybridization signals were scanned with a Generation Ⅲ array scanner and analyzed by Imagequant 5.0 software. Typical differentially expressed genes were further verified by realtime quantitative PCR. Results:There were 330 differentially expressed genes between M6 and M8 cells. In the highmetastasis M8 cells, 178 genes were upregulated and 152 genes were downregulated compared to the lowmetastasis M6 cells, with 43 extremely upregulated and 49 extremely downregulated. The differentially expressed genes were mainly associated with cell proliferation, indicating these genes might be related to the inhibition of M6 cells. Other differentially expressed genes included those associated with the regulation of gene expression and signal transduction, indicating these genes might be correlated with tumor metastasis. Conclusion:cDNA microarray shows an advantage in identifying genes associated with metastasis of osteosarcoma. In M8 subset of MG63 osteosarcoma cells，43 genes are upregulated and 49 genes are downregulated, which may be related with metastasis of osteosarcoma.
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