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Nerve growth factor promotes proliferation of human pancreatic cancer cell line MIA PaCa-2
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Abstract:
Objective:To investigate the effect of nerve growth factorβ(NGF-β) on the proliferation and cell cycle of human pancreatic cancer MIA PaCa-2 cells. Methods:MIA PaCa2 cells were treated with different concentrations of NGFβ and K252a (inhibitor of NGF-β receptor TrKA) alone or in combination. Clone forming rate, proliferation, and cell cycle of MIA PaCa2 cells treated with different strategies were examined by clone formation assay, MTT, and flow cytometry, respectively. Results:NGF-β significantly increased the clone formation and proliferation of MIA PaCa-2 cells (P<0.05, P<0.01). K252a significantly inhibited the proliferation of MIA PaCa-2 cells (P<0.05), while NGF-β combined with K252a had no significant effect on the proliferation of MIA PaCa-2 cells. NGF-β arrested MIA PaCa-2 cell cycle in S phase, K252a arrested cell cycle in G0/G1 phase, and NGF-β combined with K252a arrested cell cycle in S phase. Conclusion:NGF-β can enhance the proliferation of pancreatic carcinoma MIA PaCa-2 cells.
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Project Supported:
Project supported by the Doctor Foundation of Rizhao People’s Hospital (No. 080901)
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