Mobile website
Prompt Report
Isolation and identification of large cell lung cancer stemlike cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To isolate and identify lung cancer stemlike cells from large cell lung cancer specimens, so as to lay a foundation for immunotherapy targeting lung cancer stem cells. Methods: Cancer cells were isolated and cultured from large cell lung cancer specimens using serumfree medium, and a large cell lung cancer cell line was established. Lung cancer subset cells expressing different degrees of CD44 and (or) CD90 were sorted by flow cytometry. Biologic characters of the different subsets were studied by single cell clone formation assay, plat colony formation assay, cell sphere formation assay, and Xray radiation sensitive assay. Results: Highly purified primary cancer cells were successfully cultured from large cell lung cancer specimens using primary cell culture technology, and the cells, named LC006, could be passaged for more than 30 generations. Flow cytometry results showed that 1.0% of LC006 cells expressed stronger CD44（CD44++LC006 cells）than the main population, which was weakly positive for CD44 (CD44+LC006 cells). CD44++LC006 cells had stronger colongy formation ability and could form holoclones and cell spheres; however, CD4+LC006 cells formed neither holoclones nor cell spheres, indicating that the CD44++LC006 cells contained cancer stem cells. When CD44 and CD90 antibodies were costained, LC006 cells could be individuated into 4 subsets, i.e. CD44+CD90+ (12.8%), CD44+CD90-(86.3%), CD44++CD90+(0.4%), and CD44++CD90-(0.5%) LC006 cells; CD44++CD90+LC006 cells showed strongest cell sphere formation ability and Xray resistant ability than the other three subsets. These results indicated that CD90 might be used as another cancer stemlike cell marker for large cell lung cancer. Conclusion: CD44++CD90+LC006 cells are successfully isolated and identified from large cell lung cancer, which may be the cancer stemlike cells of large cell lung cancer.
Keywords:
Project Supported:
Project su目的：从大细胞肺癌组织中分离、鉴定肺癌干细胞样细胞，为进一步研究靶向肺癌干细胞的免疫治疗奠定基础。方法：利用无血清培养基从大细胞肺癌组织中分离、培养肿瘤细胞并建立大细胞肺癌细胞系。流式细胞仪分选表达CD44和（或）CD90的不同肺癌细胞亚群，通过单细胞克隆形成实验、平板集落形成实验、细胞球形成实验及X射线敏感实验研究不同肺癌细胞亚群的生物学特性。结果：应用原代细胞培养技术成功地从大细胞肺癌组织中培养出高纯度的原代肿瘤细胞，命名为LC006细胞，其传代次数可达30代以上。流式细胞仪分析显
Clc Number:
R734.2; Q279
